Roles of Calcium, Calmodulin and Calcineurin in Hydrogen Peroxide-Induced Cytotoxicity in Huaman Neuroblastoma SH-SY5Y Cells

Abstract

The accumulation of reactive oxygen species (ROS) leads to oxidative stress
generation and induction in cell death. Recent evidence has demonstrated the role of calcium dysregulation in pathological process of neurodegenerative diseases. However, the contribution of calcium transducer, calmodulin and calcineurin in oxidative stress-induced neuron cells death has not been elucidated. This study was aimed to investigate the timedependent effect of oxidative stress on activation in calcium-dependent cell death process. The non-radical ROS, hydrogen peroxide (H2O2) was used to induce oxidative stress in
neuroblastoma SH-SY5Y cells. The results showed that 200 μM H2O2 treatment for 24 hours significantly decreased cell viability. The levels of 32 kDa-cleavage form of calcineurin was significantly increased at 2 hours of H2O2 treatment. Moreover, H2O2-treated for 8, 12, and 24 hours significantly increased the levels of calmodulin. This finding might emphasize the involvement of calcineurin and calmodulin in oxidative stress-induced cytotoxicity in neuroblastoma cells.