Transcriptome analysis in ovary of female banana shrimp, Fenneropenaeus merguiensis: A functional study of nudel as a potential gene involved in the ovarian development

Abstract

Control of ovarian maturation is an important one of problems in the shrimp aquaculture. Several genes involved in control of crucial ovarian development process like vitellogenesis in female crustaceans. In this study, the ovary at each ovarian developmental stage of female banana shrimp, Fenneropenaeus merguiensis, was analyze for its transcriptome with Illumina HiSeq2500 platform. The nudel (ndl) was identified for study of characterization and the role of FmNudel in vitellogenesis of female banana shrimp. The result suggested that the FmNudel expression was significantly highest in ovary compared to other tissues. Its expression was significantly increased in previtellogenic stage (stage 0), then dropped in the other stages. The silencing of FmNudel was performed by nudel-dsRNA injection on day 10 resulted in significant decrease of vitellogenin (Vg) expression in ovary.