Efficiency of erythrosine gel, anthocyanin gel and nano titanium dioxide - mediated photodynamic therapy by blue light on killing Candida albicans - in vitro

Manuswee Tanapoomchai

Abstract


The purposes were to evaluate the effect of erythrosine (Er) gel, anthocyanin (cyanidin, Cy) geland Er + Cy gel stimulated by blue light (BL) and to evaluate nano-titanium dioxide (TiO2) on killing Candida albicans biofilms in PDT in vitro. Candida albicans biofilms were prepared, test and controlgroups including Er 220 μM, 220 μM+ 1%TiO2, 440 μM, 440 μM+ 1%TiO2, Cy 202 μM, 202
μM+ 1% TiO2, 404 μM, 404 μM+ 1%TiO2, Er 440 μM + Cy 404 μM and Er 440 μM + Cy 404 μM and 1%TiO2 + BL. Test groups were stimulated by BL (λ 420-480 nm, energy 15 J/cm2) for 1 minute and control groups without light. After 1, 3 and 6 hours, the biofilms were prepared for CFU counted. Results showed that the Er gel 440 μM +1%TiO2 is the most effective group on killing C. albicans when
stimulated by BL (p-value <0.001).


Keywords


Photodynamic therapy; Candida albicans; erythrosine; nano-titanium dioxide

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