Quantitative Determination of Mangiferin Isolated from Leaves of Mangifera indica L. Variety Nam Doc Mai using HPTLC and Its DPPH Scavenging Activity

วิไลพรรณ ลีปรีชานนท์


Mangiferin (2-beta-D-glucopyranosyl-1,3,6,7-tetrahydroxyxanthen-9-one) was isolated from Leaves of Mangifera indica L. variety Nam Doc Mai. It showed a potent scavenging activity on DPPH redical with an IC50 value of 6.38 mg/ml. A simple and cost-effective high- performance thin-layer chromatographic (HPTLC) method was developed and validated for quantitative determination of mangiferin. The method was performed on silica gel 60 F254 pre-coated plate with ethyl acetate-acetone-formic acid-water 8:2:1:1 (v/v) as mobile phase. Detection and quantitation was achieved by densitometric scanning at the wavelength of 320 nm. The results from validation method indicated that the proposed HPTLC method provided a good linearity at the range of 1.0 to 3.0 mg/band, accuracy (107.89 ± 0.29 %), precision (intra-day RSD 0.51-1.71% and inter-day RSD 1.52%), and specificity (sharp peak at Rf value of 0.48).


Mangiferin; HPTLC; DPPH radical

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Andreu GP, Delgado R, Velho J, et al. Mangifera indica L. extract (Vimang) inhibits Fe2+ -citrate-induced lipoperoxidation in isolated rat liver mitochondria. Pharmacol Res 2005; 51: 427-435.

Ankli A, Reich E, Steiner M. Rapid high-performance thin-layer chromatographic method for detection of 5% adulteration of black cohosh with Cimicifuga foetida, C. heracleifolia, C. dahurica, or C. americana. J AOAC Int 2008; 91(6): 1257-1264.

Beltran AE, Alvarez Y, Xavier FE, et al. Vascular effects of the Mangifera indica L. extract (Vimang). Eur J Pharmacol 2004; 499: 297-305.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 1995; 28: 25-30.

Dar A, Faizi S, Naqvi S, et al. Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biol Pharm Bull 2005; 28(4): 596-600.

Garcia D, Leiro J, Delgado R, et al. Mangifera indica L. extract (Vimang) and mangiferin modulate mouse humoral immune responses. Phytother Res 2003; 17(10): 1182-1187.

Garrido G, Delgado R, Lemus Y, et al. Protection against septic shock and suppression of tumor necrosis factor alpha and nitric oxide production on macrophages and microglia by a standard aqueous extract of Mangifera indica L. (Vimang®) role of mangiferin isolated from the extract. Pharmacol Res 2004; 50: 165-172.

Hernandez P, Rodriguez PC, Delgado R, et al. Protective effect of Mangifera indica L. polyphenols on human T lymphocytes against activation-induced cell death. Pharmacol Res 2007; 55: 167-173.

Jutiviboonsuk A, Sardsaengjun C. Mangiferin in leaves of three thai mango (Mangifera indica L.) varieties. IJPS 2010; 6(3): 122-129.

Kapadi C, Patel B, Katkar K, Suthar A, Chauhan VS. A quantitative estimation of 14-deoxy-11, 12-didehydroandrographolide in Andrographis paniculata by HPTLC. Int J Pharm Sci 2010; 2(4): 49-52.

Leiro JM, Alvarez E, Arranz JA, et al. In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor-α and transforming growth factor-β genes. Biochem Pharmacol 2003; 65: 1361-1371.

Ling LT, Yap SA, Radhakrishnan AK, et al. Standardised Mangifera indica extract is an ideal antioxidant. Food Chem 2009; 113: 1154-1159.

Martinez G, Giuliani A, Leon OS, et al. Effect of Mangifera indica L. extract (QF808) on protein and hepatic microsome peroxidation. Phytother Res 2001; 15(7): 581-585.

Miura T, Ichiki H, Hashimoto I, et al. Antidiabetic activity of a xanthone compound, mangiferin. Phytomedicine 2001; 8(2): 85-87.

Nong C, He W, Fleming D, et al. Capillary electrophoresis analysis of mangiferin extracted from Mangifera indica L. bark and Mangifera persiciformis C.Y. Wu et T.L. Ming leaves. J Chromatogr B 2005; 826: 226-231.

Reich E, Schibli A, Widmer V. HPTLC methods for identification of green tea and green tea extract. J Liq Chromatogr R T 2006; 29: 2141-2151.

Ribeiro SMR, Barbosa LCA, Queiroz JH, et al. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem 2008; 110: 620-626.

Sanchez GM, Re L, Giuliani A, et al. Protective effects of Mangifera indica L. extract, mangiferin and selected antioxidants against TPA-induced biomolecules oxidation and peritoneal macrophage activation in mice. Pharmacol Res 2000; 42(6): 565-573.

Sarkar A, Sreenivasan Y, Ramesh GT, et al. β-d-glusoside suppresses tumour necrosis factor-induced activation of nuclear transcription factor κB but potentiates apoptosis. J Biol Chem 2004; 279: 33768- 33781.

Schibli A, Reich E. Modern TLC: a key technique for identification and quality control of botanicals and dietary supplements. J Planar Chromatogr 2005; 18: 34-38.

Widmer V, Handloser D, Reich E. Quantitative HPTLC analysis of artemisinin in dried Artemisia annua L.: a practical approach. J Liq Chromatogr R T 2007; 30: 2209-2219.

Yoshimi N, Matsunaga K, Katayama M, et al. The inhibitory effects of mangiferin, a naturally occurring glucosylxanthone, in bowel carcinogenesis of male F344 rats. Cancer Lett 2001; 163: 163-170.


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